KMID : 0545120120220091296
|
|
Journal of Microbiology and Biotechnology 2012 Volume.22 No. 9 p.1296 ~ p.1300
|
|
Development of a Qualitative Dose Indicator for Gamma Radiation Using Lyophilized Deinococcus
|
|
Lim Sang-Yong
Song Du-Sup Joe Min-Ho Kim Dong-Ho
|
|
Abstract
|
|
|
The feasibility of using Deinococcus showing strong resistance to both desiccation and ionizing radiation as a dose indicator of gamma radiation exposure was evaluated. Three Deinococcus strains having different levels of radiation resistance, Deinococcus radiodurans (DRD), Deinococcus radiopugnans (DRP), and the DRD pprI mutant (DRM), were selected to develop an appropriate dose indicator for a broad range of exposures. DRD, DRP, and DRM cultures with different numbers of cells [~107 to 103 colony forming units (CFU)/100 ¥ìl] were lyophilized and subjected to various doses of gamma radiation to determine a critical dose that inhibited bacterial growth completely. Finally, a combination of DRD at ~107 and ~106 CFU, DRP at ~105 CFU, and DRM at ~104 CFU successfully indicated exposure to 5, 10, 20, and 30 kGy of gamma radiation, respectively. This study shows the possibility of developing a qualitative indicator of radiation exposure using Deinococcus.
|
|
KEYWORD
|
|
dose indicator, Deinococcus, pprI, gamma radiation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|